Transgenesis: An efficient tool in mulberry breeding
نویسندگان
چکیده
منابع مشابه
Transgenesis and Genomics in Molecular Breeding of Forage Plants
Forage plant breeding has been largely based on phenotypic selection following sexual recombination of natural genetic variation found between and within ecotypes. Advances in plant genetic manipulation over the last 15 years have provided convincing evidence that these powerful technologies can complement and enhance plant breeding programs. Significant progress in the establishment of the met...
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The silkworm, Bombyx mori is a lepidopteron insect by virtue of its economic importance for over a century has been used in many genetical experiments in several countries, especially China, India, Japan, Soviet Russia, Korea and many Southeast Asian and European countries. Many of the economic traits of this insect are known to be controlled by large number of polygenes. The silkworm varieties...
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The widespread use of fish as model systems is still limited by the mosaic distribution of cells transiently expressing transgenes leading to a low frequency of transgenic fish. Here we present a strategy that overcomes this problem. Transgenes of interest were flanked by two I-SceI meganuclease recognition sites, and co-injected together with the I-SceI meganuclease enzyme into medaka embryos ...
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Microinjection of DNA is now the most widespread method for generating transgenic animals, but transgenesis rates achieved this way in higher mammals are extremely low. To address this longstanding problem, we used lentiviral vectors carrying a ubiquitously active promoter (phosphoglycerate kinase, LV-PGK) to deliver transgenes to porcine embryos. Of the 46 piglets born, 32 (70%) carried the tr...
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ژورنال
عنوان ژورنال: African Journal of Biotechnology
سال: 2013
ISSN: 1684-5315
DOI: 10.5897/ajb2013.12090